我司科研团队今年第三篇SCI论文被权国际威杂志收载

发布时间:2013/10/6 8:03:44 浏览:1315

我司关于磷酸化蛋白质特异性检测的最新研究成果:Improved staining of phosphoproteins with high sensitivity in polyacrylamide gels using Stains-All,被最新一期《Electrophoresis》(影响因子:3.256)收载,论文通讯作者为我司金利泰教授。在该论文中,本研究团队改进了Stain All检测方法,可在1小时内完成操作,能特异性检测低至0.5-1.0 ng的微量磷酸化蛋白质。改进的Stain All检测法灵敏度比原方法提高了120倍,与目前最常用的Pro-Q Diamond方法一致,具有广泛的应用前景。

链接:http://onlinelibrary.wiley.com/doi/10.1002/elps.201300328/abstract

文章题目:Improved staining of phosphoproteins with high sensitivity in polyacrylamide gels using Stains-All

摘要:An improved Stains-All (ISA) staining method for phosphoproteins in SDS-PAGE was described. Down to 0.5–1 ng phosphoproteins (α-casein, β-casein or phosvitin) can be successfully selectively detected by ISA stain, which is approximately 120-fold higher than that of original Stains-All (SA) stain, but is similar to that of commonly used Pro-Q Diamond stain. Furthermore, unlike the original SA protocol that was time-consuming and light-unstable, ISA stain could be completed within 60 min without resorting to protect the gels from light during the whole staining procedure. According to the results, it is concluded that ISA stain is a rapid, sensitive, specific and economic staining method for a broad application to the research of phosphoproteins.

  


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