热烈祝贺我司科研团队论文被《Electrophoresis》杂志接收

发布时间:2013/9/20 17:57:24 浏览:1929

918日,最新一期的生物大分子检测领域权威杂志《Electrophoresis》(影响因子:3.256)刊登了我司科研团队最新研究成果:Fluorescent staining of protein in sodium dodecyl sulfate polyacrylamide gels by salicylaldehyde azine,论文的通讯作者为我司金利泰教授。在该论文中,本研究团队采用水杨醛吖嗪做为蛋白质荧光标记物,在聚丙烯酰胺凝胶上建立了一种新型蛋白质检测方法。该方法灵敏高,可在1小时内完成操作,灵敏度达0.2-0.4 ng/mm2,高于目前最常用的SYPRO Ruby染色方法数倍,可与银染相媲美。同时该方法价格相对低廉,线性范围广,质谱兼容性好,可广泛应用于高通量蛋白质组学的研究。

链接:http://onlinelibrary.wiley.com/doi/10.1002/elps.201300241/abstract

文章题目:Fluorescent staining of protein in sodium dodecyl sulfate polyacrylamide gels by salicylaldehyde azine

摘要:As a non-covalent fluorescence probe, in this study, salicylaldehyde azine (SA) was introduced as a sensitive fluorescence-based dye for detecting proteins both in 1-D and 2-D polyacrylamide electrophoresis gels. Down to 0.2 ng of single protein band could be detected within 1 h, which similars to that of glutaraldehyde (GA)-silver stain, but approximately four times higher than that of SYPRO Ruby fluorescent stain. Furthermore, comparative analysis of the MS compatibility of SA stain with SYPRO Ruby stain indicated that SA stain is compatible with the downstream of protein identification by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Additionally, the probable mechanism of the SA stain was investigated by molecular docking. The results demonstrated that the interaction between SA and protein was mainly contributed by hydrogen bonding and hydrophobic forces.

  


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